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pig interferon α  (Cusabio)


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    Structured Review

    Cusabio pig interferon α
    Figure 1 Time course of serological indicators in minipigs infected with HEV from different species. All experiments were performed using plasma samples. A ALT levels. The normal range was 31–58 IU/L. B AST levels. The normal range was 32–84 IU/L. C Lipase levels. The normal range was < 100 IU/L. D <t>IFN-α</t> levels, E IFN-γ levels, and F seroconversion. The cut-off value was calculated as 0.5 plus the mean absorbance of the non-reactive control. *p < 0.05, ***p < 0.001
    Pig Interferon α, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pig+interferon+%CE%B1/Pig+Interferon+%CE%B1%2CIFN-%CE%B1+ELISA+Kit/pm38982477-73-18-25
    Average 92 stars, based on 4 article reviews
    pig interferon α - by Bioz Stars, 2026-10
    92/100 stars

    Images

    1) Product Images from "Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains."

    Article Title: Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains.

    Journal: Veterinary research

    doi: 10.1186/s13567-024-01337-3

    Figure 1 Time course of serological indicators in minipigs infected with HEV from different species. All experiments were performed using plasma samples. A ALT levels. The normal range was 31–58 IU/L. B AST levels. The normal range was 32–84 IU/L. C Lipase levels. The normal range was < 100 IU/L. D IFN-α levels, E IFN-γ levels, and F seroconversion. The cut-off value was calculated as 0.5 plus the mean absorbance of the non-reactive control. *p < 0.05, ***p < 0.001
    Figure Legend Snippet: Figure 1 Time course of serological indicators in minipigs infected with HEV from different species. All experiments were performed using plasma samples. A ALT levels. The normal range was 31–58 IU/L. B AST levels. The normal range was 32–84 IU/L. C Lipase levels. The normal range was < 100 IU/L. D IFN-α levels, E IFN-γ levels, and F seroconversion. The cut-off value was calculated as 0.5 plus the mean absorbance of the non-reactive control. *p < 0.05, ***p < 0.001

    Techniques Used: Infection, Clinical Proteomics, Control

    Related Articles

    Clinical Proteomics:

    Article Title: Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains
    Article Snippet: .. To demonstrate the plasma levels of interferon-α (IFN-α) and interferon-γ (IFN-γ) and seroconversion, ELISA was performed using a Pig Interferon α, IFN-α ELISA Kit (CSB-E07328p; Cusabio Technology, Houston, TX, USA), a Porcine IFN-gamma ELISA Kit (ES9RB; Thermo Fisher Scientific, Waltham, MA, USA) and an HEV ELISA 4.0v (veterinary) (63,541–096; MP Biomedicals, Irvine, CA, USA). ..

    Article Title: Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains.
    Article Snippet: .. To demonstrate the plasma levels of interferon-α (IFNα) and interferon-γ (IFN-γ) and seroconversion, ELISA was performed using a Pig Interferon α, IFN-α ELISA Kit (CSB-E07328p; Cusabio Technology, Houston, TX, USA), a Porcine IFN-gamma ELISA Kit (ES9RB; Thermo Fisher Scientific, Waltham, MA, USA) and an HEV ELISA 4.0v (veterinary) (63,541–096; MP Biomedicals, Irvine, CA, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains
    Article Snippet: .. To demonstrate the plasma levels of interferon-α (IFN-α) and interferon-γ (IFN-γ) and seroconversion, ELISA was performed using a Pig Interferon α, IFN-α ELISA Kit (CSB-E07328p; Cusabio Technology, Houston, TX, USA), a Porcine IFN-gamma ELISA Kit (ES9RB; Thermo Fisher Scientific, Waltham, MA, USA) and an HEV ELISA 4.0v (veterinary) (63,541–096; MP Biomedicals, Irvine, CA, USA). ..

    Article Title: Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains.
    Article Snippet: .. To demonstrate the plasma levels of interferon-α (IFNα) and interferon-γ (IFN-γ) and seroconversion, ELISA was performed using a Pig Interferon α, IFN-α ELISA Kit (CSB-E07328p; Cusabio Technology, Houston, TX, USA), a Porcine IFN-gamma ELISA Kit (ES9RB; Thermo Fisher Scientific, Waltham, MA, USA) and an HEV ELISA 4.0v (veterinary) (63,541–096; MP Biomedicals, Irvine, CA, USA). ..



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    Image Search Results


    Figure 1 Time course of serological indicators in minipigs infected with HEV from different species. All experiments were performed using plasma samples. A ALT levels. The normal range was 31–58 IU/L. B AST levels. The normal range was 32–84 IU/L. C Lipase levels. The normal range was < 100 IU/L. D IFN-α levels, E IFN-γ levels, and F seroconversion. The cut-off value was calculated as 0.5 plus the mean absorbance of the non-reactive control. *p < 0.05, ***p < 0.001

    Journal: Veterinary research

    Article Title: Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains.

    doi: 10.1186/s13567-024-01337-3

    Figure Lengend Snippet: Figure 1 Time course of serological indicators in minipigs infected with HEV from different species. All experiments were performed using plasma samples. A ALT levels. The normal range was 31–58 IU/L. B AST levels. The normal range was 32–84 IU/L. C Lipase levels. The normal range was < 100 IU/L. D IFN-α levels, E IFN-γ levels, and F seroconversion. The cut-off value was calculated as 0.5 plus the mean absorbance of the non-reactive control. *p < 0.05, ***p < 0.001

    Article Snippet: To demonstrate the plasma levels of interferon-α (IFNα) and interferon-γ (IFN-γ) and seroconversion, ELISA was performed using a Pig Interferon α, IFN-α ELISA Kit (CSB-E07328p; Cusabio Technology, Houston, TX, USA), a Porcine IFN-gamma ELISA Kit (ES9RB; Thermo Fisher Scientific, Waltham, MA, USA) and an HEV ELISA 4.0v (veterinary) (63,541–096; MP Biomedicals, Irvine, CA, USA).

    Techniques: Infection, Clinical Proteomics, Control

    Proinflammatory cytokines in BAL fluid of pigs intratracheally inoculated with wtTX98 or lvTX98. Conventional 6-week-old influenza-naive pigs were intratracheally inoculated with 7.5 log 10 TCID 50 of wtTX98 (●, n = 15) or lvTX98 (■, n = 15) in 3 mL of PBS. Three pigs per group were euthanized at different time points, and BAL fluid was collected. After removal of BAL cells, cell-free BAL fluid was concentrated 20 times for cytokine analysis. (A) Bioactive IFN-α/β titers as determined in a CPE reduction assay with VSV on MDBK cells. (B) IFN-α ELISA titers. (C) IFN-β ELISA titers. (D) Bioactive IL-6 titers as determined in a proliferation assay with B9 cells. Dots represent log 2 -transformed cytokine titers for individual pigs; blue and orange bars represent mean values for wtTX98- and lvTX98-inoculated pigs, respectively, at each time point. Black dotted lines represent the detection limit. Results were compared between groups using 2-sided Mann-Whitney U tests. # , P < 0.1; #, P = 0.1.

    Journal: Journal of Virology

    Article Title: Pathobiology of an NS1-Truncated H3N2 Swine Influenza Virus Strain in Pigs

    doi: 10.1128/jvi.00519-22

    Figure Lengend Snippet: Proinflammatory cytokines in BAL fluid of pigs intratracheally inoculated with wtTX98 or lvTX98. Conventional 6-week-old influenza-naive pigs were intratracheally inoculated with 7.5 log 10 TCID 50 of wtTX98 (●, n = 15) or lvTX98 (■, n = 15) in 3 mL of PBS. Three pigs per group were euthanized at different time points, and BAL fluid was collected. After removal of BAL cells, cell-free BAL fluid was concentrated 20 times for cytokine analysis. (A) Bioactive IFN-α/β titers as determined in a CPE reduction assay with VSV on MDBK cells. (B) IFN-α ELISA titers. (C) IFN-β ELISA titers. (D) Bioactive IL-6 titers as determined in a proliferation assay with B9 cells. Dots represent log 2 -transformed cytokine titers for individual pigs; blue and orange bars represent mean values for wtTX98- and lvTX98-inoculated pigs, respectively, at each time point. Black dotted lines represent the detection limit. Results were compared between groups using 2-sided Mann-Whitney U tests. # , P < 0.1; #, P = 0.1.

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits used for detection of IFN-α and IFN-β in 20-times-concentrated BAL fluids were the Verikine-HS pig interferon alpha ELISA kit from PBL Assay Science (catalog no. 47100-1, lot no. 7031, detection range of 2.34 to 150 pg/mL) and the porcine IFN-β ELISA kit from Emelca Bioscience (catalog no. MBS760799-2, lot no. P0074E040, detection range of 15.625 to 1,000 pg/mL).

    Techniques: Enzyme-linked Immunosorbent Assay, Proliferation Assay, Transformation Assay, MANN-WHITNEY